Idiopathic pulmonary fibrosis diagnosis marker and application thereof

• Main Authors: ZHENG PEIYAN, CHENG ZHANGKAI, SUN SHIXUE, XUE MINGSHAN, HUANG HUIMIN, SUN BAOQING, LUO WENTING
• Format: Patent
• Language: Chinese; English
• Published: 03.02.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses an idiopathic pulmonary fibrosis diagnostic marker and application thereof. The marker comprises a transcript ENST00000529044, and the transcript ENST00000529044 can be used for According to the invention, third-generation single-molecule RNA real-time sequencing and second-generation RNA sequencing are carried out on idiopathic pulmonary fibrosis (IFP) lung tissues, it is found for the first time that the expression level of the transcript ENST00000529044 has a significant difference in the expression levels of the IFP lung tissues and normal lung tissues, and the lung idiopathic pulmonary fibrosis can be accurately and effectively detected by measuring the transcript ENST00000529044 in samples such as blood and the like. 本发明公开了特发性肺纤维化病诊断标志物及其应用。所述标志物包括转录本ENST00000529044。本发明对特发性肺纤维化(IFP)肺组织进行第三代单分子RNA实时测序和第二代RNA测序，首次发现转录本ENST00000529044的表达水平在IFP肺组织和正常肺组织中的表达水平存在显著差异，通过对血液等样本中的转录本ENST00000529044的测定，可准确有效的检测肺部特发性肺纤维化。