Fluorescent indicator substitution method-based GTP recognition tripodal anion receptor and preparation method and application thereof

• Main Authors: HATA, YOSHIMI, CAO QIANYONG
• Format: Patent
• Language: Chinese; English
• Published: 03.02.2023
• Subjects: CHEMISTRY; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PHYSICS; TESTING

The invention discloses a GTP recognition tripodal anion receptor based on a fluorescent indicator substitution method as well as a preparation method and application of the GTP recognition tripodal anion receptor. The three-legged anion receptor contains a quenching group and anion action site ammonia hydrogen and pyridinium salt positive ions. Receptor molecules and some anion fluorescence indicators can be self-assembled into a nano assembly in an aqueous solution, and meanwhile, the fluorescence of the indicators is quenched. GTP is added into the system, so that the fluorescent indicator is released from the assembly through a replacement competition relationship, and meanwhile, the fluorescence is recovered. The detection limit of the system on GTP fluorescence recognition reaches 9.49 nM. 本发明公开了一种基于荧光指示剂置换法GTP识别三足阴离子受体及其制备方法和应用。该三足阴离子受体含有猝灭基团和阴离子作用位点氨氢和吡啶盐正离子。受体分子与一些阴离子荧光指示剂在水溶液中能自组装成纳米组装体，同时使指示剂荧光猝灭。在该体系中加入GTP，通过置换竞争关系使荧光指示剂从组装体中释放出来，同时使荧光恢复。该系对GTP荧光识别的检测限达到9.49nM。