Method for promoting volume expansion of tetrastigma hemsleyanum in-vitro tuberous roots

• Main Authors: SONG YUETAO, ZHANG JIAMEI, YANG QINGBAO, HONG SENRONG
• Format: Patent
• Language: Chinese; English
• Published: 03.02.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention discloses a method for promoting volume expansion of in-vitro root tubers of tetrastigma hemsleyanum, and particularly relates to the technical field of detoxification and in-vitro induction of plant root tubers. The method specifically comprises the following steps: (1) disinfecting a tetrastigma hemsleyanum stem, embedding into calcium alginate gel beads, and then transferring into a first culture medium for pre-culture; and (2) adding the pre-cultured stem segment obtained in the step (1) into a PVS2 solution for loading, then adding a PVS2 solution dispersed with carbon nanotubes and having the same volume as the stem segment for dehydration, then carrying out ultralow-temperature freezing and thawing, and carrying out recovery culture, thus obtaining the volume-expanded tetrastigma hemsleyanum in-vitro root tuberous root of tetrastigma hemsleyanum in-vitro root tuberous root of tetrastigma hemsleyanum in-vitro root tuberous root of tetrastigma hemsleyanum. According to the method, the tetra