Improved method for polysaccharide quantification

The present invention provides a method for measuring the concentration and/or amount of one or more polysaccharides in a test sample comprising or consisting of: (a) acid hydrolysis of the test sample with hydrochloric acid and trifluoroacetic acid; (b) chromatographic separation of the test sample...

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Bibliographic Details
Main Authors GIANELLI, CARLO, MICOLI FRANCESCA, RUSSO MAURIZIO M
Format Patent
LanguageChinese
English
Published 31.01.2023
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SEPARATION
TESTING
TRANSPORTING
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Summary:The present invention provides a method for measuring the concentration and/or amount of one or more polysaccharides in a test sample comprising or consisting of: (a) acid hydrolysis of the test sample with hydrochloric acid and trifluoroacetic acid; (b) chromatographic separation of the test sample hydrolyzed in step (a); and (c) determining the concentration and/or amount of one or more polysaccharides based on the data produced in step (b), as well as a treated test sample and a kit for use thereof. 本发明提供一种用于测量测试样品中的一种或多种多糖的浓度和/或量的方法，其包括或由以下步骤组成：(a)用盐酸和三氟乙酸对测试样品的酸水解；(b)步骤(a)的水解的测试样品的色谱分离；和(c)基于步骤(b)中产生的数据，确定一种或多种多糖的浓度和/或量，还一起提供了经处理的测试样品和用于其使用的试剂盒。
Bibliography:Application Number: CN202180042391