Immune biosensor for detecting PEDV (porcine epidemic diarrhea virus) as well as preparation method and application thereof

• Main Authors: LI ZHILI, GUO JINYUE, LUO YUHANG, WEN FENG, CHI SHIHONG, SU LANGJU, HUANG ZONGYANG, MA GUANGMIAO
• Format: Patent
• Language: Chinese; English
• Published: 31.01.2023
• Subjects: CHEMISTRY; COMPOUNDS THEREOF; INORGANIC CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; NON-METALLIC ELEMENTS; PHYSICS; TESTING

The invention relates to an immune biosensor for detecting PEDV (Porcine Epidemic Diarrhea Virus) as well as a preparation method and application of the immune biosensor, and relates to the field of rapid pathogen detection. The immune biosensor comprises an electrode base material, a graphene oxide layer, a chitosan layer and a porcine epidemic diarrhea virus monoclonal antibody layer, the graphene oxide layer, the chitosan layer and the porcine epidemic diarrhea virus monoclonal antibody layer are sequentially stacked and covered on the electrode base material. The immune biosensor has excellent sensitivity, accuracy and specificity on the detection of PEDV (Porcine Epidemic Diarrhea Virus). 本发明涉及一种用于检测PEDV的免疫生物传感器及其制备方法和应用，涉及病原快速检测领域。该免疫生物传感器包括电极基材、氧化石墨烯层、壳聚糖层、猪流行性腹泻病毒单克隆抗体层；氧化石墨烯层、壳聚糖层、猪流行性腹泻病毒单克隆抗体层依次层叠覆盖于电极基材。该免疫生物传感器针对PEDV的检测具有优异的灵敏性、精确性和特异性。