Nanometer magnetic bead sorting method for epithelial-derived tumor cells

The invention relates to a nano magnetic bead sorting method for epithelium-derived tumor cells. The method comprises the following specific operation modes: (1) adding carboxy l ic aci d activated magnetic nanoparticles into 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide fo...

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Bibliographic Details
Main Authors ZHU YU, ZOU HUAN, LAN JINGTING, HUANG MIN, LAN JIANQIANG, XU CONG
Format Patent
LanguageChinese
English
Published 24.01.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a nano magnetic bead sorting method for epithelium-derived tumor cells. The method comprises the following specific operation modes: (1) adding carboxy l ic aci d activated magnetic nanoparticles into 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide for activation; adding an anti-EpCam monoclonal antibody, an anti-Ki-67 monoclonal antibody and/or an anti-pan-CK monoclonal antibody, reacting at room temperature, and cleaning with PBS after the reaction is completed to obtain a magnetic bead precursor; adding bovine serum albumin to seal non-specific adsorption sites to prepare functional magnetic beads; (3) cell sorting: taking the functional magnetic beads, adding the tumor cell sample, sorting by using a magnetic frame, collecting tumor cells for counting, and detecting the proportion of the tumor cells by using a flow cytometer. The functional magnetic beads are prepared through a convenient method, the functional magnetic beads and tumor cell samples are rapi
Bibliography:Application Number: CN202211349510