Multi-dimensional liver cancer precise molecular typing risk assessment method based on CTDNA expression

• Main Author: WANG SIZHEN
• Format: Patent
• Language: Chinese; English
• Published: 20.01.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATIONTECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING ORPROCESSING OF MEDICAL OR HEALTHCARE DATA; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a multi-dimensional liver cancer precise molecular typing risk assessment method based on CTDNA expression. The method comprises the following steps: S1, collecting a liver cancer precise molecular sample of a subject; s2, preparing a specific primer and a probe for detecting or measuring the methylation state or level of the SEPT9 gene in the DNA of the tested sample, and determining the expression molecular typing rate of the gene data in the liver cancer precise molecular sample; s3, performing mass-to-charge ratio (mass-to-charge ratio, m/z) separation according to chronological order or spatial position, and giving molecular weight, molecular formula and structural information of the compound; s4, determining the multi-dimensional liver cancer precise molecular typing and/or survival risk of the subject according to the molecular weight, molecular formula and structural information of the gene data in the sample, thereby realizing comprehensive and accurate analysis, realizing abs