Method for adapting virus in chicken embryo fibroblasts

The invention relates to a method for adapting viruses in chick embryo fibroblasts, and belongs to the technical field of biology. The invention provides a method for adapting viruses in chick embryo fibroblasts, which is characterized in that the metabolic level of the chick embryo fibroblasts in a...

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Bibliographic Details
Main Authors TIAN DAYONG, AN QI, RUAN JUNCHENG, FU ZHENFANG
Format Patent
LanguageChinese
English
Published 20.01.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a method for adapting viruses in chick embryo fibroblasts, and belongs to the technical field of biology. The invention provides a method for adapting viruses in chick embryo fibroblasts, which is characterized in that the metabolic level of the chick embryo fibroblasts in a culture maintenance stage is greatly reduced by controlling the serum content of a cell maintenance fluid to be 0.5-2%, the fluid change period of the cell maintenance fluid to be 2-6 days and the cell density of the chick embryo fibroblasts to be 6-12 * 10 < 5 >/mL, so that the survival rate of the viruses in the chick embryo fibroblasts is increased, and the survival rate of the viruses in the chick embryo fibroblasts is increased. The in-vitro survival time of the chick embryo fibroblasts is prolonged (the maintenance time of the chick embryo fibroblasts is prolonged from 12 days before optimization to 60-72 days after optimization), so that after the measles wild strains are infected with the chick embryo fibr
Bibliography:Application Number: CN202211513116