Method for generating human pancreatic beta cells by inducing IPS cell differentiation in vitro

The invention discloses a method for generating human pancreatic beta cells by inducing IPS cell differentiation in vitro. The method specifically comprises the following steps: (1) preparing IPS cells, a culture medium and an induced differentiation agent; (2) carrying out induced differentiation o...

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Bibliographic Details
Main Author WANG XINJUAN
Format Patent
LanguageChinese
English
Published 13.01.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a method for generating human pancreatic beta cells by inducing IPS cell differentiation in vitro. The method specifically comprises the following steps: (1) preparing IPS cells, a culture medium and an induced differentiation agent; (2) carrying out induced differentiation on the culture medium A and the induced differentiation agent A to obtain cells A; (3) carrying out induced differentiation on the culture medium B and an induced differentiation agent B to obtain cells B; (4) carrying out induced differentiation on the culture medium C and an induced differentiation agent C to obtain cells C; and (5) carrying out induced differentiation on the culture medium D and the induced differentiation agent D to obtain the pancreatic beta cells. The invention relates to the technical field of bioengineering, and particularly provides a culture medium suitable for directional culture of IPS cells, so as to ensure that the IPS cells are efficiently and directionally differentiated into the pan
Bibliography:Application Number: CN202211295021