Method for reducing heat production in pichia pastoris fermentation process

• Main Authors: DU HAN, ZHAO JIANGBIAO, DING MANSHENG, FAN MIN
• Format: Patent
• Language: Chinese; English
• Published: 13.01.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

In order to solve the technical problems of high heat production and high requirements on refrigeration equipment in the process of producing foreign proteins in a large scale by recombinant fermentation of pichia pastoris, the invention provides a pichia pastoris fermentation process with low heat production, which is characterized in that fermentation tank culture comprises a glycerol culture stage and an induction stage, wherein in the induction stage, a mixed carbon source of methanol and sorbitol is used for fed-batch feeding, the heat yield of pichia pastoris fermentation is reduced through the carbon source function of sorbitol instead of methanol, and the problem that energy consumption is too high in the process of pichia pastoris fermentation expression of foreign proteins is solved. 为解决毕赤酵母重组发酵规模化生产外源蛋白过程中产热量大、制冷设备要求高的技术问题，本公开提供一种产热量小的毕赤酵母发酵工艺，其特征在于发酵罐培养包括甘油培养阶段和诱导阶段，其中所述诱导阶段以甲醇和山梨醇混合碳源进行流加补料，通过山梨醇代替甲醇的碳源功能降低毕赤酵母发酵的产热量，解决毕赤酵母发酵表达外源蛋白过程中能耗过高的问题。