Analysis method for measuring content of hydroxypropyl beta cyclodextrin in human plasma by liquid chromatography-mass spectrometry
The invention provides an analysis method for determining the content of hydroxypropyl beta-cyclodextrin in human plasma through liquid chromatography-mass spectrometry. The analysis method comprises the following steps: preparing a working solution; pre-treating a sample; and a liquid chromatograph...
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Main Authors | , , , |
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Format | Patent |
Language | Chinese English |
Published |
06.01.2023
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Subjects | |
Online Access | Get full text |
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Summary: | The invention provides an analysis method for determining the content of hydroxypropyl beta-cyclodextrin in human plasma through liquid chromatography-mass spectrometry. The analysis method comprises the following steps: preparing a working solution; pre-treating a sample; and a liquid chromatography-mass spectrometry method is adopted for detection. The method comprises the following steps: preparing and analyzing a hydroxypropyl beta cyclodextrin stock solution, an internal standard substance stock solution, a standard curve working solution, a quality control sample working solution, an internal standard substance working solution, a standard curve sample solution, a quality control sample solution and a quantitation limit sample solution; the detection extraction recovery rate, the matrix effect, the matrix stability, the accuracy, the precision, the stability after treatment, the hemolysis effect, the hyperlipemia effect, the whole blood stability, the plasma selectivity and the like of the analysis meth |
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Bibliography: | Application Number: CN202111565025 |