Method for detecting fatty acid compounds in MCF-7 cell metabolite

• Main Authors: HUANG YUXIANG, LIU XIAOJUAN, QI HUANYANG, LEE JI-KEUN, LIU CONG, ZHANG JIAYUAN
• Format: Patent
• Language: Chinese; English
• Published: 03.01.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for detecting fatty acid compounds in MCF-7 cell metabolites, which comprises the following steps: carrying out synchronization treatment on MCF-7 cells in a good culture state, then quenching the cells by adopting a quenching agent, and collecting the cells; performing cell disruption on the collected cells to obtain cell endogenous substances; removing proteins in the endogenous substances, and extracting by using petroleum ether, dichloromethane or normal hexane as an extraction reagent to obtain the MCF-7 cell metabolite; and detecting the fatty acid compounds in the MCF-7 cell metabolite by adopting a gas chromatography-mass spectrometry method. The method can be used for detecting the fatty acid compounds in various MCF-7 cell metabolites, and is of great significance to related researches on intracellular fatty acid metabolism. 本发明公开了一种检测MCF-7细胞代谢物中脂肪酸类化合物的方法，对培养状态良好的MCF-7细胞进行同步化处理，随后采用淬灭剂淬灭细胞，收集细胞；对收集到的细胞进行细胞破碎，获得细胞内源性物质；除去内源性物质中的蛋白质后，用石油醚、二氯甲烷或正己烷作为提取试剂提取得到MCF-7细胞代谢物；