Trace protein quantitative detection reagent and detection method

• Main Authors: ZHANG XIAO, YANG XINGYU, CAO JUNFENG, XIA QINGJIE, SHENG ZIYI
• Format: Patent
• Language: Chinese; English
• Published: 30.12.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a trace protein quantitative detection reagent. The trace protein quantitative detection reagent comprises a DNA fragment marked by an affinity molecule A and an affinity molecule B, the affinity molecule B can mark a protein to be detected, and the affinity molecule A and the affinity molecule B can be specifically combined with each other; the sequence of the DNA fragment is as shown in SEQ ID NO. 1. The invention also provides a method for quantitatively detecting the trace protein, the affinity molecule A is used for marking the to-be-detected protein, the affinity molecule B is used for marking the artificially synthesized DNA fragment, the characteristic that the affinity molecule A and the affinity molecule B can be specifically combined with each other is utilized, the DNA fragment is detected by a quantitative PCR technology, and the amount of the to-be-detected protein is reflected through the relative amount of DNA. The method is quick, simple and convenient to operate, and c