Efficient rubber tree secondary body embryo generation method and application thereof

• Main Authors: CHENG LIAN, HUANG HUASUN, XU ZHENGWEI, WU RIZHI, HUANG TIANDAI, NARIKAMI, YANG XIUZHU, DAI XUEMEI, PENG SUNA, GU XIAOCHUAN
• Format: Patent
• Language: Chinese; English
• Published: 23.12.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention provides a rubber tree secondary body embryo efficient generation method and application thereof, and belongs to the technical field of plant cell engineering. The method comprises the following steps: cutting a cotyledon of a somatic cell embryo into an embryo block with the length * width of 2-4mm * 2-4mm, firstly attaching a far axial surface of the embryo block to a callus induction culture medium for inoculation, and then attaching a near axial surface of the embryo block with embryogenic callus to a somatic embryo differentiation culture medium for inoculation. It is found that the number of embryonic calluses of embryonic calluses cultured by a limited embryonic mass far-axial-surface-attached callus induction culture medium is remarkably increased, and the number of embryonic masses of secondary somatic embryos differentiated by a limited embryonic mass near-axial-surface-attached somatic embryo differentiation culture medium and the number of the secondary somatic embryos are remarkably