Efficient rubber tree secondary body embryo generation method and application thereof

The invention provides a rubber tree secondary body embryo efficient generation method and application thereof, and belongs to the technical field of plant cell engineering. The method comprises the following steps: cutting a cotyledon of a somatic cell embryo into an embryo block with the length *...

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Main Authors CHENG LIAN, HUANG HUASUN, XU ZHENGWEI, WU RIZHI, HUANG TIANDAI, NARIKAMI, YANG XIUZHU, DAI XUEMEI, PENG SUNA, GU XIAOCHUAN
Format Patent
LanguageChinese
English
Published 23.12.2022
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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Summary:The invention provides a rubber tree secondary body embryo efficient generation method and application thereof, and belongs to the technical field of plant cell engineering. The method comprises the following steps: cutting a cotyledon of a somatic cell embryo into an embryo block with the length * width of 2-4mm * 2-4mm, firstly attaching a far axial surface of the embryo block to a callus induction culture medium for inoculation, and then attaching a near axial surface of the embryo block with embryogenic callus to a somatic embryo differentiation culture medium for inoculation. It is found that the number of embryonic calluses of embryonic calluses cultured by a limited embryonic mass far-axial-surface-attached callus induction culture medium is remarkably increased, and the number of embryonic masses of secondary somatic embryos differentiated by a limited embryonic mass near-axial-surface-attached somatic embryo differentiation culture medium and the number of the secondary somatic embryos are remarkably
Bibliography:Application Number: CN202211140538