Refining method of myocardial cells

• Main Authors: NISHIMOTO MASAYUKI, WATANABE KOZO, TANAKA REI, YOSHIDA YOSHINORI, KONDO SHIGERU
• Format: Patent
• Language: Chinese; English
• Published: 02.12.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present invention provides a method for producing a cell population comprising cardiac muscle cells, the method comprising: (1) a step for bringing a cell population comprising cardiac muscle cells and cells other than cardiac muscle cells into contact with a histone deacetylase inhibitor, the cell population being obtained by culturing pluripotent stem cells in a culture medium for cardiac muscle cell differentiation; and (2) a step for culturing the cell population. 本发明提供一种制造包含心肌细胞的细胞群的方法，其包括：(1)使包含心肌细胞和心肌细胞以外的细胞的细胞群与组蛋白去乙酰化酶抑制剂接触的步骤，该细胞群是将多能干细胞在心肌细胞分化用培养基中培养得到的；和(2)培养该细胞群的步骤。