Formula and preparation process of cyperus esculentus tissue culture technology culture medium

• Main Authors: CHEN LIYU, BI XIULI, LU ZHANYUAN, ZHANG DEJIAN, REN YONGFENG, ZHAO XIAOQING, FAN QINGWEN, DU YUNQIN, ZHANG XIANGQIAN, QIAO XUEJING, SOHN DONG-HYUN, YU PING, SUN YUXIN, WANG JINGSHUN, LI JUAN, CHENG YUCHEN, SU YUNHUA, WANG JIANGUO, XUE CHUNLEI, LIU CHUNYAN, ZOU FEI
• Format: Patent
• Language: Chinese; English
• Published: 02.12.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FERTILISING; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PLANTING; SOWING; TRAPPING

The invention discloses a formula and a preparation process of a culture medium for a cyperus esculentus tissue culture technology, and aims to solve the problems that a new variety of cyperus esculentus is difficult to cultivate by using a conventional hybridization technology, the problem of variety degeneration is easy to occur when the cyperus esculentus is propagated by using tubers for a long time and the cyperus esculentus culture medium formula is less, so that the cyperus esculentus is extremely easy to have a browning phenomenon in stem tip culture. According to the invention, large-scale production of seedlings and germplasm innovation of excellent varieties can be realized through tissue and mutagenesis technologies. In the plant tissue culture process, sterile buds of cyperus esculentus serve as explants, an optimal cluster bud proliferation culture medium is adopted, tissue culture seedling propagation production is achieved through the three culture steps of stem tip differentiation culture, pr