Improvements in DNA synthesis yields

• Main Authors: ROTHWELL PAUL, PORTER NEIL, KERSH DIKLA
• Format: Patent
• Language: Chinese; English
• Published: 25.11.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to an improved method for the synthesis of deoxyribonucleic acids (DNAs), in particular for the cell-free enzymatic synthesis of DNA, preferably in large-scale or industrial scale, with increased yield and/or with increased efficiency. The present invention entails the use of nucleotide complexes in which the nucleotides bind to a mixture of divalent and monovalent cations. Preferably, the divalent cation may be magnesium or manganese. 本发明涉及一种用于合成脱氧核糖核酸(DNA)的改进方法，尤其是用于DNA的无细胞酶促合成，优选以大规模或工业规模，该方法具有提高的的产量和/或具有提高的效率。本发明需要使用核苷酸复合物，其中核苷酸与二价和一价阳离子的混合物结合。优选地，所述二价阳离子可以是镁或锰。