Use of CAS9 protein from bacterial pasteurella pneumophila

• Main Authors: ABRAMOVA MARIYA VLADIMIROVNA, ANDREEVA JANNE V, ALTAMONOVA TATYANA OLEGOVNA, SEVERINOV KONSTANTIN VLADIMIROVICH, ZUBUK THOMAS I, POBEGAROV GENNADII EMIL, MUSHAROVA OLGA SERGEEVNA, FEDOROVA IRINA VLADIMIROVNA, KHODORKOVSKY, MICHAEL, A, SHMAKOV SERGEY ALEKSANDROVICH, ARCHENEV ANDREY NIKOLAEVICH, SERKOVA, PETR ALEXANDROVICH, GORYANIN ILYA IVANOVICH, ALTAMONOVA DINA NIKOLAEVNA, VASILYEVA ALLA ALEXANDROVNA
• Format: Patent
• Language: Chinese; English
• Published: 25.11.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention describes a novel bacterial nuclease from the CRISPR-Cas9 system of the bacterial Pasteurella pneumophila (P.pneumophila), and the use of the nuclease for producing a strictly specific double strand cleavage in a DNA molecule. The nuclease has unique properties and can be used to alter genomic DNA sequences in cells of single-cell or multicellular organisms. The present invention thus increases the universality of available CRISPR-Cas9 systems, enabling the use of different Cas9 nuclease variants to cleave genomes or plasmid DNA in various organisms in a large number of specific sites and/or under different conditions. 本发明描述了来自细菌嗜肺巴斯德杆菌(P.pneumotropica)的CRISPR-Cas9系统的新型细菌核酸酶，以及所述核酸酶用于在DNA分子中产生严格特异性的双链切割的用途。所述核酸酶具有独特的性质，并且可以用于改变单细胞或多细胞生物体的细胞中的基因组DNA序列。本发明因此增加了可用CRISPR-Cas9系统的普适性，使得能够使用不同的Cas9核酸酶变体，以在大量特异性位点中和/或在不同条件下，切割各种生物体中的基因组或质粒DNA。