Method for promoting lipopeptide production by co-culturing bacillus amyloliquefaciens and recombinant yarrowia lipolytica

• Main Authors: WON YOUNG-JIN, QIAO BIN, HOU ZHENGJIE, CHENG JINGSHENG, BAI SONG
• Format: Patent
• Language: Chinese; English
• Published: 22.11.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for promoting lipopeptide production by co-culturing bacillus amyloliquefaciens and recombinant yarrowia lipolytica. The method comprises the following steps: (1) seed culture; (2) fermentation culture: inoculating the secondary seeds of the recombinant yarrowia lipolytica into a fermentation culture medium according to OD600 of 0.2-0.4, and culturing for 6-8 hours under the conditions that the temperature is 28-32 DEG C and the speed is 180-200 rpm; and inoculating secondary seeds of bacillus amyloliquefaciens according to the condition that the OD600 is 0.2-0.4, and fermenting under the conditions that the temperature is 28-32 DEG C and the speed is 180-200rpm. The method can effectively increase the yield of lipopeptide, and is simple and low in cost. In a fermentation culture medium containing the treated kitchen garbage, bacillus amyloliquefaciens and recombinant yarrowia lipolytica can be mutually beneficial and symbiotic, starch and lipid in the kitchen garbage are conv