SNP (Single Nucleotide Polymorphism) molecular marker for selecting egg weight of hen

• Main Authors: LI JINGYI, CAI JINPING, LI SHIJUN, MA ZHUOWANG, RONG LI, YANG SENDONG, JIA ZIJIA, RUAN HONGJI, YANG KE, GONG YANCHANG, NAN JIUHONG, SHENG ZHEYA, PENG XIULI, FENG YANPING, ZHU GUIYU
• Format: Patent
• Language: Chinese; English
• Published: 15.11.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for selecting the egg weight of a hen and application of the SNP molecular marker, two SNP molecular markers associated with the egg weight of the hen are screened on a hen genome through candidate gene association analysis and a PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) technical method, and SNP loci are respectively positioned at the 64135953rd and 67657474th basic groups of a chromosome 1. The polymorphism of the SNPs sites is obviously associated with the egg weight of hens, and the SNP molecular marker can be independently used for selecting and improving the egg weight of individual hens. 本发明公开了一种用于选择母鸡产蛋蛋重的SNP分子标记及其应用，通过候选基因关联分析和限制性内切酶长度多态性（PCR-RFLP）技术方法，在母鸡基因组上筛选到2个与母鸡产蛋蛋重相关联的SNP分子标记，SNP位点分别位于1号染色体第64135953位和第67657474位碱基。这些SNPs位点的多态性与母鸡的产蛋蛋重显著关联，所述的SNP分子标记可以单独使用，用于选择和改良母鸡个体的产蛋蛋重。