Method for detecting aminophylline residues in animal tissues and application

• Main Authors: PAN YANHUI, PENG SHUDE, YUAN ZHONGCE, WANG LIN, LI MINGYANG, LIANG SHANSHAN, HU JIAN, HOU QIANG, HUO LUMAN, CUI SHAN, LI NING, LI YI, SONG WANG, DONG JIEQIONG, LEI SHUHAN, LI YINGQI, XIANG AILI, ZHOU XIN, SUN MINGQI, WANG XUESONG, ZHANG LIFANG
• Format: Patent
• Language: Chinese; English
• Published: 04.11.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for detecting aminophylline residues in animal tissues and application of the method. The method comprises the following steps: treating a sample to be detected by using an acetonitrile-water solution, and collecting supernate containing aminophylline after vortex treatment, ultrasonic treatment and centrifugation; carrying out solid-phase extraction, concentration, constant volume and filtration on the supernate to obtain a to-be-tested solution; and analyzing the to-be-detected solution by using high performance liquid chromatography-tandem triple quadrupole mass spectrometry to realize qualitative and quantitative detection of aminophylline. According to the detection method disclosed by the invention, the extraction and recovery rate of aminophylline in low-concentration animal tissues is high in the preparation step of the to-be-detected liquid, the response signal of a detection peak is effectively improved in the purification process, the cleanliness of the to-be-detect