Tissue culture method of semi-mangrove plant Myopporum bontioides

The invention discloses a tissue culture method of semi-mangrove plant Myopporum bontioides, and belongs to the field of plant tissue culture. The method comprises the following steps: (1) explant selection and disinfection: taking tender Myopporum bontioides axillary buds as explants, and carrying...

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Bibliographic Details
Main Authors DU ZHAOKUI, HONG XIAOLING, YANG DANG, LI JUNMIN, QIU ZHIMIN
Format Patent
LanguageChinese
English
Published 04.11.2022
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Summary:The invention discloses a tissue culture method of semi-mangrove plant Myopporum bontioides, and belongs to the field of plant tissue culture. The method comprises the following steps: (1) explant selection and disinfection: taking tender Myopporum bontioides axillary buds as explants, and carrying out disinfection treatment; (2) callus formation: inoculating the disinfected Myopporum bontioides axillary buds into an induction culture medium, and inducing to generate calluses; (3) induced formation of cluster buds: transferring the calluses into a differential medium, and inducing to form the cluster buds; (4) subculture of cluster buds: cutting off single cluster buds, transferring the single cluster buds into a subculture medium, and culturing the single cluster buds into rootless seedlings; (5) root induction formation: transferring the rootless seedlings subjected to subculture into a rooting culture medium, and inducing rooting; (6) domesticating the tissue culture seedlings; and (7) transplanting tissue
Bibliography:Application Number: CN202211025327