Bone cell lysis buffer-based/vesicle-based hydrogel as well as preparation method and application thereof

• Main Authors: ZHANG XINGDING, ZHENG LIYUAN, ZHOU DISHENG, ZHENG YONGJIANG
• Format: Patent
• Language: Chinese; English
• Published: 01.11.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biomedical materials, in particular to bone cell lysis buffer-based/vesicle-based hydrogel as well as a preparation method and application thereof. The invention provides bone cell lysis buffer-based/vesicle-based hydrogel capable of being used for repairing bone injury in a tumor microenvironment as well as a preparation method and application of the bone cell lysis buffer-based/vesicle-based hydrogel. According to the method, a large number of bone cells capable of regulating and promoting osteogenesis activity can be obtained; and cell active components with a regulating function in bone cells can be effectively fixed and preserved. According to the invention, a stable oscillating fluid shear force is applied to the bone cells in an oscillating culture manner, and the oscillating frequency, amplitude, fluid volume and fluid loading time of the carbon dioxide oscillating incubator are controlled to obtain the optimal stimulation condition for activating the bo