Protease mutant

• Main Authors: BIAN FURONG, LI JUAN, LIU YANJIE, YAN TING, ZHAN MILIN, ZHU JIDONG
• Format: Patent
• Language: Chinese; English
• Published: 28.10.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a protease mutant. The mutant has amino acid substitution on the 11th site corresponding to SEQ ID NO: 1; and the mutant has protease activity. The amino acid sequence of the protease mutant is as shown in SEQ ID NO: 2. Besides, through scientific mutation point scheme design and a large number of mutant screening experiments, the fermentation activity of the obtained protease mutant in host cells is remarkably improved compared with that of parent protease, the production cost of the protease is reduced in industrial production, the productivity of protease products of factories is improved, and the production cost of the protease is reduced. The method provides a basis for wide application in different fields, and has good application prospect and industrial value. 本发明公开了一种蛋白酶突变体，所述突变体在对应于SEQ ID NO：1的第11位上具有氨基酸的取代；且所述突变体具有蛋白酶活性。本发明所述蛋白酶突变体的氨基酸序列如SEQ ID NO：2所示。另外，本发明通过科学的突变点方案设计及大量的突变体筛选实验，获得的蛋白酶突变体在宿主细胞中的发酵活力与其亲本蛋白酶相比显著增加，有利于在工业化生产中降低该酶的生产成本，提高工厂的蛋白酶产品产能，为其广泛应用于不同领域提供基础，具有良好的应用前景和产业价