Aflatoxin biosensor and preparation method thereof

• Main Authors: CAO QIRONG, ZHANG RAN, LYU YUZHU, LI MINGHAN, NIE XIAOTONG, JIANG XIAOTENG, ZHU YUNXUAN, LI YIFU, CHEN DONGDONG, GUO YUANZHEN, HU SHAOLUN, ZHAO YANG
• Format: Patent
• Language: Chinese; English
• Published: 25.10.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses an aflatoxin biosensor and a preparation method thereof, and relates to the technical field of biosensor manufacturing. The aflatoxin biosensor comprises heme, a TMB (tetramethylbenzidine) color developing agent, an aptamer, a Trigger chain and hairpin probes HP1 and HP2, and the preparation method of the aflatoxin biosensor comprises the following steps: step S1, designing a DNA (deoxyribonucleic acid) chain; step S2, preprocessing the hairpin probes HP1 and HP2; step S3, preparation of a three-chain DNA probe; and S4, synthesizing the biosensor. The aflatoxin biosensor disclosed by the invention can be matched with heme and a TMB (Tetramethylbenzidine) color developing agent, and can develop color when aflatoxin B1 is detected, so that a detector can judge whether aflatoxin B1 exists or not according to whether the color appears or not, and a corresponding electronic display device does not need to be matched; meanwhile, colors with different depths can be shown according to the cont