Internal standard assisted petal-shaped SERS (Surface Enhanced Raman Scattering) nanoprobe as well as preparation method and application thereof

• Main Authors: ZHANG SHUSHENG, LYU MENGYA, FAN JIAYI, CHEN SHENG, ZHANG WENTING
• Format: Patent
• Language: Chinese; English
• Published: 21.10.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses an internal standard assisted petal-shaped SERS (Surface Enhanced Raman Scattering) nanoprobe as well as a preparation method and application thereof, and the internal standard assisted petal-shaped SERS nanoprobe is a core-molecule-shell structure gold nanoflower probe consisting of a gold nanosphere core, an internal standard modification molecule, a petal-shaped gold nanoshell and a recognition molecule 4-nitrothiophenol; the internal standard modification molecule is a Raman signal molecule with a nitrile group, alkynyl or azide functional group. A Raman signal molecule is used as an internal standard modification molecule to modify the gold nanosphere core, a corrected working curve is good in reproducibility and small in error, and the reliability of a detection result is improved; by growing a layer of petal-shaped gold nano shell on a molecular layer of internal standard modification molecules, the SERS activity inside and outside the SERS substrate is improved, and the surface