Method for detecting cell migration ability

• Main Authors: ZHANG KANGJIN, HUANG LIJING, DA QI, ROTHSCH
• Format: Patent
• Language: Chinese; English
• Published: 21.10.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention belongs to the technical field of biology, and particularly relates to a method for detecting cell migration ability. The invention discloses a method for detecting cell migration ability. The method comprises at least one of S1 to S3: S1: staining cells; s2, scratching is carried out by adopting an occupying scratching method; s3, culturing the cells for 3-16 hours by adopting a culture medium containing mitomycin C, wherein the final concentration of the mitomycin C is 0.8-4.5 mu g/mL; or using a culture medium containing mitomycin C to culture the cells for 18-22 hours, wherein the final concentration of the mitomycin C is 0.3-0.7 mu g/mL; by dyeing the cells, the accuracy of quantitative analysis is improved, and the time required by quantitative analysis is shortened; according to the method, the stable and uniform scratches can be obtained through scratching by adopting an occupying scratching method; the method comprises the following steps: culturing cells by adopting a culture medium co