Method for serum-free large-scale culture of rotavirus by using bioreactor

• Main Authors: CUN YINA, FAN QIQI, HENG XIE, LIU YALING, CHEN HONGBO, ZHOU JIAN, ZHAO HONGLING, ZHANG ZEHENG, YONG GUOSHENG, SHI JINLI, BAO XIAOLIN, CHEN YU, LIU BOCHUAN
• Format: Patent
• Language: Chinese; English
• Published: 21.10.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of preparation of virus inactivated vaccines, in particular to a method for serum-free large-scale culture of rotaviruses by using a bioreactor. The method for serum-free large-scale culture of rotavirus by using the bioreactor comprises the following steps: serum-free culture of Vero cells by using the bioreactor, virus inoculation and culture. According to the method, pancreatin is supplemented every day, proliferation of viruses is promoted, rotavirus liquid is harvested in a continuous harvesting mode, and the yield of the viruses is greatly increased. Serum in a traditional culture medium is complex in component, difficult to detect and high in potential risk, and a serum-free culture medium is clear in component and free of animal source component addition. By adopting the content of the invention, when the large-scale production of the bioreactor is carried out, the labor force, the space demand and the pollution risk are reduced, and meanwhile, the potentia