Immune composition for detecting circulating tumor cells

• Main Authors: LIANG QIAN, LIU ZHONGHUA, WANG PING, LIANG MI, ZHANG ZHENZHEN, JIN WEI, GAO JIEFENG, WANG GUOQIANG, LI PENGFEI
• Format: Patent
• Language: Chinese; English
• Published: 04.10.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides an immune composition for detecting circulating tumor cells, high-sensitivity detection of CTC (circulating tumor cells) surface protein is realized through the immune reagent composition, and the composition comprises an antibody, nano microspheres, a polyfunctional group compound and a detectable marker. The immunoreagent composition is mainly specifically combined with a CTC surface antigen through an antibody, the antibody is connected with a nano-microsphere through a linear or dendritic polyfunctional group compound, and the nano-microsphere is connected with a detectable marker through the linear or dendritic polyfunctional group compound. The whole system realizes multiple amplification of a cell surface protein signal through transfer of the nano-microspheres, so that high-sensitivity detection of a target antigen on the surface of the CTC is realized. 本发明提供一种用于检测循环肿瘤细胞的免疫组合物，通过所述免疫试剂组合物实现对CTC表面蛋白的高灵敏度检测，组合物包含抗体、纳米微球、多官能团化合物、可检测标记物。免疫试剂组合物主要是通过抗体与CTC表面抗原特异性结合，所述抗体通过线状或者树枝状的多官能团