Methods for predicting ancestral virus sequences and uses thereof

• Main Authors: ZINN ERIC, VANDENBERGHE LUK H
• Format: Patent
• Language: Chinese; English
• Published: 04.10.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

Methods of predicting ancestral viral sequences and uses thereof are provided. A method for predicting an ancestor sequence of a virus or a portion thereof is described. Also described are predicted ancestral sequences of adeno-associated virus (AAV) capsid polypeptides. The present disclosure also provides methods of gene transfer and methods of vaccinating a subject by administering a target antigen operably linked to an AAV capsid polypeptide. 提供了预测祖先病毒序列的方法及其用途。描述了用于预测病毒或其部分的祖先序列的方法。还描述了腺伴随病毒(AAV)壳体多肽的经预测的祖先序列。本公开还提供了基因转移的方法和对受试者接种疫苗的方法，其通过施用可操作连接于AAV壳体多肽的靶抗原进行。