Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes

• Main Authors: BRINKERHOFF HENRY DRAKE, DEKKER, CORNELIS
• Format: Patent
• Language: Chinese; English
• Published: 30.09.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

A method (100) for translocating a peptide (11) through a nanopore (50) wherein the method (100) comprises translocating a peptide (11) in the presence of an oligonucleotide translocating enzyme (20) wherein the peptide (11) is comprised by a peptide-oligonucleotide complex (10) wherein the peptide (11) is linked to an oligonucleotide (13) wherein the oligonucleotide translocating enzyme (20) is associated with the oligonucleotide (13) during at least partial translocation. 一种用于使肽(11)易位穿过纳米孔(50)的方法(100)，其中方法(100)包括在寡核苷酸易位酶(20)的存在下使肽(11)易位，其中肽(11)由肽-寡核苷酸复合物(10)包含，其中肽(11)连接至寡核苷酸(13)，其中寡核苷酸易位酶(20)在至少部分易位期间与寡核苷酸(13)相关联。