Construction method of methylnaphthoquinone-7 storage space in bacillus subtilis

• Main Authors: SONG HAO, WANG QINGMEI, LI FENG, SUN PANXING, ZHANG JUNQI, XIAO LIN, CHEN YARU
• Format: Patent
• Language: Chinese; English
• Published: 30.09.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a construction method of a methylnaphthoquinone-7 storage space in bacillus subtilis, which comprises the following steps: introducing a key enzyme DGAT gene synthesized by triacylglycerol TAG into engineering bacillus subtilis by utilizing a heterologous expression technology and a genetic engineering means to construct a recombinant plasmid I so as to realize TAG synthesis in bacillus subtilis; therefore, the storage space of the methylnaphthoquinone-7 in the bacillus subtilis is expanded; or the key gene for synthesizing the triacylglycerol TAG and the lipid droplet protein TadA form a combination and are introduced into engineering bacillus subtilis to construct a recombinant plasmid II, so that the TAG and the lipid droplet protein in the bacillus subtilis are combined and assembled to form the lipid droplet LDs, and the storage space of the methylnaphthoquinone-7 in the bacillus subtilis is expanded, thereby promoting the synthesis of the methylnaphthoquinone-7. 本发明公开了一种枯草芽孢杆菌中甲基