Microscope and method for forming microscopic images with extended depth of field

• Main Authors: ILIOPOULOS PAVLOS, KARANIN ILYA, SEO, CHEONG SOO, GAIDUK ALEXANDER, CHO, GYOUNG IL, SON JIN YOUNG
• Format: Patent
• Language: Chinese; English
• Published: 27.09.2022
• Subjects: OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; PHYSICS

The invention relates to a microscope and a method for forming a microscopic image having an extended depth of field. The method produces a microscopic image having an extended depth of field by means of a microscope. The microscope includes an image sensor including pixels arranged as a matrix formed by lines. In one step of the method, a plurality of microscopic frames of the sample are acquired while changing the focal position (z). And obtaining the microscopic frames line by line. The focal position (z) is varied during acquisition of individuals of the microscopic frame. In a further step, a portion of an individual of the acquired line is identified. These portions sharply image the sample. The identified portion of the line is composed so as to form a microscopic image of the sample having an extended depth of field. The invention further relates to a microscope. 本发明涉及一种用于形成具有扩展景深的显微图像的显微镜和方法，该方法借助于显微镜来产生具有扩展景深的显微图像。该显微镜包括图像传感器，该图像传感器包括布置为由线形成的矩阵的像素。在该方法的一个步骤中，在改变焦点位置(z)的同时获取样本的多个显微帧。逐线获取显微帧。在获取显微帧的个体