Internal reference gene of red sage root anther and application of internal reference gene

• Main Authors: YANG RUIWU, LIN LI, ZHANG YUNSONG, DENG XUEXUE, ZHANG LI, SHANG YUKUN, LIAO JINQIU, CHAI SONGYUE, JIANG YUANYUAN, WANG LONG
• Format: Patent
• Language: Chinese; English
• Published: 27.09.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an internal reference gene of a salvia miltiorrhiza anther. The internal reference gene is an Sm073914 gene and/or an Sm083165 gene. According to the invention, the Sm073914 and/or Sm083165 genes are/is taken as the reference genes of the radix salviae miltiorrhizae anther qRT-PCR to normalize the expression level of the target gene, so that the current situation that the radix salviae miltiorrhizae anther qRT-PCR does not have the reference genes is solved. 本发明公开了一种丹参花药的内参基因，所述内参基因为Sm073914基因和/或Sm083165基因。本发明以Sm073914和/或Sm083165基因作为丹参花药qRT-PCR的内参基因对目标基因的表达水平进行归一化，解决了丹参花药qRT-PCR没有内参基因的现状。