Method for rapidly purifying protein through silica gel column adsorption and elution

• Main Authors: ZHENG XINGMEI, PENG CUNZHI, CHANG LILI, TONG ZHENG, XU BINGQIANG, WANG DAN
• Format: Patent
• Language: Chinese; English
• Published: 27.09.2022
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

The invention discloses a method for rapidly purifying protein through silica gel column adsorption and elution, and relates to the technical field of biology. Comprising the following steps: S1, obtaining a target protein; s2, adding a protein binding buffer solution; s3, preparing a sol column; s4, adding the rinsing liquid; s5, adding a protein eluent; and S6, detecting the recovery effect. Target protein is purified according to the principle that a silica gel column adsorbs protein in a certain buffer system, a protein sample usually contains SDS and other surfactants, NaI + NaCl + beta-mercaptoethanol is used as a protein binding buffer solution, inhibition of the surfactants on silica gel adsorption protein can be relieved, absolute ethyl alcohol is selected as a protein rinsing solution, and the protein rinsing solution is used for rinsing protein. The protein loss caused by rinsing can be greatly reduced, the protein can be effectively eluted from the silica gel column by using a protein eluent such