Efficient animal mycoplasma culture medium and preparation method thereof

• Main Authors: XIONG QIYAN, WANG JIA, ZHANG LEI, WEI YANNA, YU YANFEI, LIU MAOJUN, SHAO GUOQING, FENG ZHIXIN, XIE XING, XIE QINGYUN
• Format: Patent
• Language: Chinese; English
• Published: 20.09.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides an efficient animal mycoplasma culture medium and a preparation method thereof, and belongs to the field of animal medicine. According to the efficient animal mycoplasma culture medium, FH and C4bp are added into a KM2 culture medium. The preparation method of the culture medium comprises the following step: adding sterile FH and C4bp into a KM2 culture medium to obtain the culture medium. By adopting the animal mycoplasma culture medium disclosed by the invention, the CCU50 value of the animal mycoplasma can be remarkably increased, the vaccine generation cost is remarkably reduced, and the immune effect is improved. 本发明提供一种高效动物支原体培养基及其制备方法，属于动物医学领域。该高效动物支原体培养基，是在KM2培养基中添加FH和C4bp。本发明培养基的配制方法，包括如下步骤：在KM2培养基中添加无菌FH和C4bp，得到所述培养基。采用本发明动物支原体培养基能够显著提高动物支原体的CCU50值，显著降低疫苗生成成本和提高免疫效果。