Method for improving doubling efficiency of corn haploid by using gibberellin

• Main Authors: ZHANG LIANG, CHEN CHUANYONG, FU XIUYI, ZHANG XUEYUAN, WU SHANSHAN, CHEN CHEN, ZHAO JIURAN, ZHANG HUASHENG, DUAN MINXIAO, GUO CHENG'EN, PENG YANKE, ZHANG CHUNYUAN, WANG YUANDONG
• Format: Patent
• Language: Chinese; English
• Published: 20.09.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; TRAPPING

The invention discloses a method for improving corn haploid doubling efficiency by using gibberellin, and belongs to the field of breeding. According to the corn haploid doubling method, gibberellin is externally applied in the young embryo doubling process, the colchicine treatment doubling rate can be effectively increased, the haploid doubling powder scattering rate and the maturing rate are greatly increased, and therefore the DH production efficiency is improved. A feasible scheme is provided for improving the doubling efficiency of the haploid immature embryo with the colchicine effect, and the efficiency of a haploid breeding technology can be greatly improved. 本发明公开了一种利用赤霉素提高玉米单倍体加倍效率的方法，属于育种领域。本发明所提供的玉米单倍体加倍的方法通过在幼胚加倍过程中外施赤霉素，可有效提高秋水仙素处理加倍率，大大提高单倍体加倍散粉率和结实率，从而提高DH生产效率。为提高秋水仙素作用的单倍体幼胚加倍效率提供了一个可行的方案，可大大提升单倍体育种技术的效率。