Variant nucleic acid libraries for adenosine receptors

• Main Authors: GARG, PANKAJ, TABIBIAZZA, RAY, SATO, AARON, LIU QIANG
• Format: Patent
• Language: Chinese; English
• Published: 16.09.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided herein are methods and compositions related to an adenosine A2A receptor library having a nucleic acid encoding a scaffold comprising an adenosine A2A binding domain. The adenosine A2A receptor libraries described herein encode immunoglobulins, which include antibodies and single domain antibodies. The libraries described herein include a diversified library comprising nucleic acids, each nucleic acid encoding a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are libraries of proteins produced when the libraries of nucleic acids are translated. Further described herein are cell libraries that express the diversified nucleic acid libraries described herein. 本文提供了与腺苷A2A受体文库相关的方法和组合物，所述腺苷A2A受体文库具有编码包含腺苷A2A结合结构域的支架的核酸。本文所述的腺苷A2A受体文库编码免疫球蛋白，所述免疫球蛋白包括抗体和单域抗体。本文所述的文库包括包含核酸的多样化文库，每个核酸编码至少一个预定参考核酸序列的预定变体。本文进一步描述了当所述核酸文库被翻译时产生的蛋白质文库。本文进一步描述了表达本文所述的多样化核酸文库的细胞文库。