Application of rose-derived glycosyl transferase RrUGT3 in biosynthesis of gastrodin

• Main Authors: LI LEYI, XU HAIXIA, LI ZHI, ZHAN YANGYANG, CHEN SHOUWEN, CAI DONGBO
• Format: Patent
• Language: Chinese; English
• Published: 16.09.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of biology, and particularly discloses application of glycosyl transferase RrUGT3 from roses in biosynthesis of gastrodin, and the sequence of the glycosyl transferase RrUGT3 is as shown in SEQ ID NO. 2. According to the present invention, the gene encoding the SEQ ID NO.2 is transferred into the Bacillus licheniformis, the p-hydroxybenzyl alcohol is added into the fermentation culture medium to perform fermentation so as to obtain the biologically fermented gastrodin, the p-hydroxybenzyl alcohol can be efficiently and specifically converted to synthesize the gastrodin, and the foundation is laid for the industrial production of the gastrodin through the microbial conversion method; 本发明属于生物技术领域，具体公开了来自于玫瑰的糖基转移酶RrUGT3在生物合成天麻素中的应用，所述的糖基转移酶RrUGT3的序列为SEQ ID NO.2所示。将编码SEQ ID NO.2的基因转入地衣芽胞杆菌中，在发酵培养基中添加对羟基苯甲醇进行发酵，即可获得生物发酵的天麻素，能高效且专一性转化对羟基苯甲醇合成天麻素，为微生物转化法工业化生产天麻素奠定基础。