Method for large-scale culture of human diploid cells for producing viral vaccines

• Main Authors: CUN YINA, FAN QIQI, HENG XIE, DUAN WEIGUO, QIAN XINGLI, LI GUOLIANG, CHEN HONGBO, ZHOU JIAN, ZHAO HONGLING, ZHANG ZEHENG, YONG GUOSHENG, SHI JINLI, CHEN YU, LIU BOCHUAN
• Format: Patent
• Language: Chinese; English
• Published: 13.09.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a method for large-scale culture of human diploid cells for producing viral vaccines. The method comprises the following steps: unfreezing and resuscitating the human diploid cells; carrying out multiplication culture on human diploid cells and preparing a cell suspension; human diploid cells are inoculated to a fixed bed type or basket type bioreactor, the human diploid cells are attached to a plurality of flaky carriers through the flaky carriers arranged in the reactor, and the large-scale human diploid cells are obtained after the human diploid cells are proliferated and propagated in the same reactor and the same culture environment. After large-scale virus inoculation and reproduction are carried out on large-scale human diploid cells, antiviral vaccines are produced on a large scale, the number of vaccines needed in the epidemic situation period is met, the human diploid cells and the antiviral vaccines have high consistency, and market requirements are met. 本发明提供一种大规模培养人二倍体细胞用于生