Fermentation medium for efficiently secreting and expressing oligopeptide protein by Escherichia coli with LPP single gene knockout or mutation and application of fermentation medium

• Main Authors: CAO CHUNLAI, LI-ZHANG WANJIN, LIANG XIONGJI, FAN CHANG, LIANG ZHIYU, YANG XIAOCHUN, HE XIUYI, LIU HEDONG, LI SUWEN, HUANG GUOZHOU, ZHOU CUI
• Format: Patent
• Language: Chinese; English
• Published: 09.09.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a fermentation medium for efficiently secreting and expressing oligopeptide proteins by escherichia coli with LPP single gene knockout or mutation and application of the fermentation medium. The fermentation medium comprises the following components: 2.55 to 3.4 g/L of citric acid, 3.5 to 4.5 g/L of magnesium sulfate heptahydrate, 15 to 36 g/L of an organic nitrogen source, 4.5 to 5.5 g/L of glycerol, 13 to 14 g/L of anhydrous monopotassium phosphate, 8 to 9 g/L of anhydrous diammonium hydrogen phosphate, 4 to 5 mg/L of vitamin B1 and 0.8 to 1.2 mL/L of trace elements. The fermentation culture medium overcomes the growth defects of mutant strains, so that the strains can grow better, the growth rate reaches the level of wild fungi, and high-density culture is realized; the secretory expression of oligopeptide protein can be obviously promoted, the density of the induced strain is stably and continuously increased, the fermentation period reaches 40 hours, and target protein in a fermen