Cyanine probe based on 4-amino-2-mercaptopyrimidine-5-carboxylic acid ethyl ester as well as preparation method and application of cyanine probe

• Main Authors: ZHANG YANPEI, QIN JUN, LIU XIAOQIAN, HUA YUWEI, ZHANG CHUCHU
• Format: Patent
• Language: Chinese; English
• Published: 09.09.2022
• Subjects: ADHESIVES; CHEMISTRY; DYES; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; LAKES; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE; MEASURING; METALLURGY; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; MORDANTS; NATURAL RESINS; ORGANIC CHEMISTRY; ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES; PAINTS; PHYSICS; POLISHES; TESTING

The invention belongs to the field of biological detection, and particularly relates to a cyanine probe based on 4-amino-2-mercaptopyrimidine-5-carboxylic acid ethyl ester as well as a preparation method and application of the cyanine probe. The preparation method comprises the following steps: firstly, preparing a CY5.5 dye, then reacting the CY5.5 dye with N-hydroxysuccinimide (NHS) to form active ester, finally reacting with 4-amino-2-mercaptopyrimidine-5-carboxylic acid ethyl ester under an alkaline condition, and separating an obtained crude product through a thin layer chromatography to obtain a final product CY5.5 O. The cyanine probe prepared by the invention has excellent optical performance and can selectively recognize lysozyme in a fluorescence change mode. 本发明属于生物检测领域，具体涉及一种基于4-氨基-2-巯基嘧啶-5-羧酸乙酯的花菁探针及其制备方法和应用。首先制备CY5.5染料，然后将CY5.5染料与N-羟基丁二酰亚胺(NHS)反应形成活性酯，最后与4-氨基-2-巯基嘧啶-5-羧酸乙酯在碱性条件下反应，所得粗品通过薄层色谱法分离，得到最终产物CY5.5O。本发明制备得到的花菁探针具有优良的光学性能并且可以通过荧光变化的方式选择性识别溶菌酶。