Abietane diterpenoid component in hyssopus officinalis extract as well as preparation method and application of bietane diterpenoid component

• Main Authors: SHI LEILING, MA GUOXU, SUN ZHAOCUI, LIU HONGYU, ZHU JUN, ABULIZI, SHATIGULI, WEI HONGYAN
• Format: Patent
• Language: Chinese; English
• Published: 06.09.2022
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; CHEMISTRY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

The invention relates to the technical field of separation and purification of the overground part of schizonepeta bracteata, in particular to Nepetatide E as well as a preparation method and application thereof, the Nepetatide E is prepared by the following steps: performing reflux extraction on the overground part of schizonepeta bracteata by using ethanol to obtain total extract of schizonepeta bracteata, extracting by using an organic solvent to obtain extract of each part, performing ethanol-water gradient elution on the extract of the n-butyl alcohol part, and concentrating to obtain the Nepetatide E. Then methanol-water gradient elution is carried out through ODS reversed-phase chromatography, a third fraction is taken and then subjected to high performance liquid chromatography elution, and then purification and separation are carried out. According to the invention, the Nepteabate E is disclosed for the first time, and the Nepteabate E disclosed by the invention has a relatively strong anti-inflammat