Application of inflammatory factor in improving proportion of positive subgroups of pedicel protein

• Main Authors: HOU XIAOQIANG, CHEN XIAOYING, SHENG HONGXIA, YUE BAOYU, CHEN YAWEI, WANG FENG, LI LIANG
• Format: Patent
• Language: Chinese; English
• Published: 30.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses an application of an inflammatory factor in improving the proportion of pingopod protein positive subgroups of a mesenchymal stem cell population. In the culture process, TNF-alpha and IFN-gamma are adopted to culture the mesenchymal stem cells, so that the proportion of PDPN positive subgroups in the mesenchymal stem cell group can be obviously increased, the mesenchymal stem cell group with high expression of the PDPN is obtained, and the cell migration ability and chemotactic ability in the mesenchymal stem cell group are obviously improved. Compared with the existing mesenchymal stem cell migration promoter si-LINC01773, the method disclosed by the invention does not need to aim at a specific target spot, and is simple to operate and wide in application range. 本发明公开了一种炎症因子在提高间充质干细胞群的平足蛋白阳性亚群比例中的应用。在培养过程中，采用TNF-α和IFN-γ培养间充质干细胞，可以明显提高间充质干细胞群中平足蛋白(podoplanin，PDPN)阳性亚群的比例，获得高表达平足蛋白的间充质干细胞群，此间充质干细胞群中的细胞迁移能力和趋化能力显著提高。相较于现有的间充质干细胞迁移促进剂si-LINC01773，本发明的方法不需要针对特异靶点，操作简单，应用范围广。