In-vitro rapid propagation method of clematis brevituba

• Main Authors: HAN XIN, WANG SHU'AN, LI YA, GAO LULU, LI LINFANG
• Format: Patent
• Language: Chinese; English
• Published: 30.08.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention discloses a rapid propagation method of clematis brevituba. The rapid propagation method comprises the following steps: S1, selecting a test material; s2, disinfecting the explant; s3, axillary bud proliferation: a proliferation culture medium is MS, 1.0-1.5 mg/L of 6-BA, 0.05 mg/L of NAA and 30 g/L of sucrose, and the pH value is 6.0-6.2; s4, axillary bud rooting, wherein a rooting culture medium is DKW, 1.0 mg/L IBA and 30 g/L cane sugar, and the pH value is 6.0-6.2. The clematis brevituba is cultivated through the technology, the rooting rate is high, roots and seedlings are strong, and the survival rate after transplanting is high. 本发明公开了一种短柱铁线莲的快速繁殖方法，包括以下步骤:S1供试材料选取；S2外植体消毒；S3腋芽增殖：增殖培养基为MS+1.0～1.5mg/L 6-BA+0.05mg/L NAA+30g/L蔗糖，pH值为6.0～6.2；S4腋芽生根：生根培养基为DKW+1.0mg/L IBA+30g/L蔗糖，pH值为6.0～6.2。通过本发明的技术对短柱铁线莲进行培育，生根率高，根苗强壮，移栽后成活率高。