Extraction method and detection method of total nitrosamines in cosmetics
The invention provides an extraction method and a detection method of total nitrosamine in cosmetics. The extraction method comprises the following steps: precisely weighing 0.5 to 1.0 g of the cosmetic, adding 50 to 150 [mu] L of a sulfamic acid aqueous solution with a concentration of 0.05 to 0.15...
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Main Authors | , , , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
26.08.2022
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Subjects | |
Online Access | Get full text |
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Summary: | The invention provides an extraction method and a detection method of total nitrosamine in cosmetics. The extraction method comprises the following steps: precisely weighing 0.5 to 1.0 g of the cosmetic, adding 50 to 150 [mu] L of a sulfamic acid aqueous solution with a concentration of 0.05 to 0.15 g/mL and 1 to 3 mL of tetrahydrofuran, and carrying out vortex oscillation for 1 to 3 min; adding a water-methanol-acetonitrile-tetrahydrofuran mixed solution until the total volume is 9mL, carrying out vortex oscillation for 1-3 minutes, carrying out ultrasonic treatment for 20-40 minutes at the temperature of 30-50 DEG C, and cooling to room temperature; adding a water-methanol-acetonitrile-tetrahydrofuran mixed solution until the total volume is 10mL, carrying out vortex oscillation for 1-3 minutes, carrying out centrifugal treatment for 5-10 minutes at the speed of 5000r/min-14000r/min, and taking supernate, so as to obtain the compound. The method disclosed by the invention is high in total nitrosamine extrac |
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Bibliography: | Application Number: CN202210462080 |