Primer group and kit for detecting SVA and EMCV based on dual NanoPCR and application of primer group and kit

• Main Authors: WANG JUN, QIN JUAN, GAO YAFEI, LI JIE, YANG YONG, JIANG GUANGZHI, SUN PEI, HE CHANGSHENG, LI LIANG, CHEN LIJUN
• Format: Patent
• Language: Chinese; English
• Published: 19.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a primer group and a kit for detecting SVA and EMCV based on dual NanoPCR and application of the primer group and the kit, and belongs to the technical field of virus detection. The primer for detecting the SVA and the EMCV through the dual NanoPCR, provided by the invention, comprises a Seneca virus A detection primer SVAF/SVA R and an encephalomyocarditis virus detection primer EMCV F/EMCV R. The method disclosed by the invention is high in specificity, high in sensitivity and simple and convenient to operate. 本发明提供了一种基于双重NanoPCR检测SVA和EMCV的引物组、试剂盒及其应用，属于病毒检测技术领域。本发明提供的双重NanoPCR检测SVA和EMCV的引物，包括塞内卡病毒A检测引物SVAF/SVA R和脑心肌炎病毒检测引物EMCV F/EMCV R。本发明所述方法特异性强、灵敏度高、操作简便。