Porcine interferon lambda4 recombinant protein as well as preparation method and application thereof

• Main Authors: LU YOUXIN, FANGPU COUNTY, PENG XUAN, XIAO XUN, FANG LIURONG, ZHOU YANRONG, LIU SHIYU, XIAO SHAOBO
• Format: Patent
• Language: Chinese; English
• Published: 19.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of biology, and relates to a porcine interferon lambda4 recombinant protein as well as a preparation method and application thereof. The amino acid sequence of the porcine interferon lambda 4 (sIFN-lambda 4) recombinant protein is as shown in SEQ ID NO: 3. The sIFN-lambda 4 fragment is connected to a plasmid to obtain a recombinant plasmid, and the nucleotide sequence of the sIFN-lambda 4 is as shown in SEQ ID NO: 4; transforming the recombinant plasmid into escherichia coli, then carrying out bacterial liquid culture, adding IPTG for induction, collecting an inclusion body, dissolving and purifying to obtain the recombinant sIFN-lambda 4 protein. The recombinant sIFN-lambda4 protein can induce gram-negative bacteria such as escherichia coli, klebsiella pneumoniae and salmonella to agglutinate so as to prevent the gram-negative bacteria from invading eukaryotic cells, and meanwhile, the recombinant sIFN-lambda4 protein can also inhibit infection of porcine epidemic