Method for rapidly enriching new coronavirus infection positive cells and application thereof

• Main Authors: ZHOU PENG, SHEN XURUI, YAN BING, SHI ZHENGLI, ZHANG WEI
• Format: Patent
• Language: Chinese; English
• Published: 12.08.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the field of biological medicine, in particular to a method for rapidly enriching new coronavirus infection positive cells and application of the method, and the method comprises the following steps: S1, labeling a new coronavirus RBD monoclonal antibody with commercial biotin to prepare a biotinylated new coronavirus RBD monoclonal antibody; s2, binding a biotinylated new coronavirus RBD monoclonal antibody to a commercial streptomycin affinity magnetic bead to obtain an antibody-magnetic bead compound; s3, incubating the prepared antibody-magnetic bead compound with cells or fully dissociated tissues for 30 minutes, carrying out magnetic separation by using a magnetic separator, washing for multiple times to remove cells which are not combined, and reducing the background; and S4, obtaining new coronavirus infection positive cells with high purity and good activity for subsequent experiments. The method is convenient and rapid, the new coronavirus infection positive cells can be obt